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DNA sequencing
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Genotyping
Custom libraries
Technology platforms
Other services
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Technology Platforms
+ ABI 3730xl+ Roche 454 GS-FLX System
+ Illumina Solexa 1G Genome Analyzer
Services
+ Whole genome shotgun sequencing+ BAC end sequencing
+ SNP discovery and resequencing
+ Large-scale EST sequencing
+ Primer walking
+ SAGE sequencing
+ Sequencing expertise
Our expertise in DNA sequencing
Dedicated in DNA sequencing services, Creative Genomics has accumulated extensive experience. Even with top-performed sequencers, problematic trace files still appear occasionally due to poly N, indel, simple repeat or secondary structure as shown below. Our vigorous quality control process ensures operators to catch and solve these abnormalities efficiently if the causes stem from technical aspects rather than from the poor qualities of submitted samples. For each individual case there will be a specific solution, the most common ones of which include sequencing the reverse stranded and/or doubling all reaction components.
Insertion and deletion
Poly N
Simple repeat
Secondary structure
The premature termination of signals during a sequencing run is usually caused by secondary structure (often in regions with a high G/C or high A/T content).