DNA sequencing
Custom cDNA Library Construction
cDNA library construction is a key step in gene discovery through EST sequencing or analysis of full-length cDNA. Using premium protocols, Creative Genomics provides highly quality services of custom cDNA library construction including standard cDNA library, large insert cDNA library and normalized cDNA library. The library construction service is of particular importance for scientists who prefer fast access to a high quality cDNA source of interest. Our technical staff will coordinate with clients to optimize the service package to meet specific project goals and manage the projects from beginning to end.
Features:
- Average cDNA insert length >1kb
- Primary clones >1 x 106
- Recombinants for plasmid library >90%
- Antibiotic resistance: Chloromycetin = 25ug/ml
- One plate (96-well) sequencing for free
- <10% redundancy after normalization
Service includes:
- Sample quality control
- 1st strand synthesis
- Adapter ligation
- Second strand synthesis and amplification
- Size selection
- Ligation into pDNR-Lib
- Transformation of ligation into E. coli DH10B
- PCR amplification of the inserts from 24 randomly picked clones to determine a percentage of recombinant clones and average insert size
Deliverables:
- Primary library stored in -20℃
- Library evaluation report, including No. of library clones, average insert size, recombinant rates, and integrality of cDNA, etc.
- Electrophoresis profiles