DNA sequencing
Genomic shotgun library construction
A whole genome shotgun library can be utilized efficiently for generating DNA sequences or identifying specific genes from total genomic DNA. Creative Genomics offers highly flexible genomic shotgun library construction services. We have extensive experience in constructing shotgun libraries of high quality from a variety of sources including bacterial, phage, Mammalian and BAC DNA.
Construction:
- High quality DNA template preparation
- Random shearing to produce a population of DNA fragments
- Ligation into a standard cloning vector
- Transformation & glycerol stock production
- QA/QC by DNA sequencing
- gDNA preparation available
- 3~4 weeks turnaround time in most cases
- Final report including original electropherograms and one plate (96 wells) sequencing result
Properties:
- Customized insert sizes: typically <10kb
- Primary Clones: 2x107 (small insert), 1x107 (large insert)
- Percentage of Recombinants: >95% (small insert), >90% (large insert)