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DNA sequencing
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Genotyping
Custom libraries
Technology platforms
Other services
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Technology Platforms
+ ABI 3730xl+ Roche 454 GS-FLX System
+ Illumina Solexa 1G Genome Analyzer
Services
+ Whole genome shotgun sequencing+ BAC end sequencing
+ SNP discovery and resequencing
+ Large-scale EST sequencing
+ Primer walking
+ SAGE sequencing
+ Sequencing expertise
BAC end sequencing
BAC (bacterial artificial chromosome) end sequencing is a powerful technique that enables the rapid and precise assembly of huge mega-base sequences of chromosomal regions in large-scale genome sequencing. It can be also utilized to identify the structural polymorphism in genome such as inversion and translocation. Creative Genomics offers a beginning-to-end solution for BAC library construction, shotgun sequencing and BAC end sequencing.
Description of Services
- Colony picking and culturing
- DNA template preparation
- BAC end DNA sequencing using universal primers
- Successful reads defined by clients (typically >500bp in both ends)
- Automated or manual basecalling
- Sequence assembly and annotation available
